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Tuesday 18 February 2014 - RDG Wales & West Event

HPTLC for Identification of Herbal Raw Material and Extracts

Eike Reich (Camag)

Synopsis

HPTLC is the most advanced form of Thin-Layer Chromatography. The use of suitable instruments for all steps of the analysis, a standardized methodology and validated methods in combination with high performance plates ensure reliable and reproducible results that are fully compliant with cGMP. The advantages of planar chromatography: simplicity, flexibility, low cost per sample, etc. are maintained. For identification of plants and plant derived products such as extracts electronic images of HPTLC fingerprints are playing a central role. They allow convenient visual comparison of multiple samples even if those were analysed on different plates. Reference images can be used to qualify data, replace expensive or difficult to obtain chemical reference substances, and pass/fail samples based on similarity or difference.

HPTLC is complementary to HPLC. It can visualize the natural variability in the chemical profile of a given species as well as the differences between desired and adulterant species. Thus it is an ideal tool for the elaboration of quality standards.

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'Blue Biotechnology' - Searching for Beauty Under the Sea: New and potential cosmetic ingredients

Joan Gonzalez (Technical Sales Manager, Infinitec)

Synopsis

Floating on the surface of the ocean without shade and not getting sunburnt, or being immersed in a high salt environment without losing moisture are some of the achievements of marine microorganisms that could be translated into very attractive cosmetic ingredients. Biological and chemical diversity in the oceans is greater than that of terrestrial ecosystems, making the sea an optimum source of new active ingredients for different industrial applications (1-5) from the pharmaceutical sector to the development of active food ingredients, and, of course, cosmetics. We associate the concept of marine biodiversity with a coral reef, teeming with different species of invertebrates, algae and fish, but the majority of diversity found in the sea belongs to the microorganisms. The focus of the cosmetic industry in the sea microbia as a source of novel ingredients is already yielding interesting results in a wide variety of applications. In summary, even though culturable bacteria represents a very small fraction of the total microbial diversity, those microorganisms that can grow in the laboratory represent a formidable reservoir of novel products for cosmetic applications

The diversity found in the chemical production of microorganisms is strongly influenced by the taxonomic diversity and the geographical and environmental characteristics of the sampling points, as well as by the methodology used in the isolation and fermentation of the isolated strains. In the process of discovering novel cosmetic ingredients of marine origin, the initial collection will be screened against selected targets, and the combination of the quality of the collection and the relevance of the screening system for the final application, will determine the chances of success of the process. Technical expertise is essential for tapping into the sea resources, and so is the knowledge of the diverse and complex needs that the cosmetic industry and its customers have.

This complexity makes symbiosis between INFINITEC and the pharmaceutical industry which share an interest in finding novel cosmetic ingredients in the sea, and the joint effort is the basis for some of the activities described in this article. The quest for biodiversity starts in the prospection, strain isolation, fermentation and screening in order to detect activities with potential application in the cosmetic industry.

 

Venue: Leigh Court, Abbots Leigh, Bristol BS8 3RA

Commencing 14.00 - 16.00 hours with light refreshments and a free buffet from 13.15 hours


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